SEMA3A
SEMA3B säätyy ylös D3 vitamiinista
SEMA3C säätyy ylös D3 vitamiinista
SEMA3D säätyy alas D3 vitamiinsita
SEMA3E indudoituu vahvimmin D3-vitamiinista
SEMA3F indusoituu vahvimmin D3 vitamiinista
SEMA3G säätyy alas D3 vitamiinista
J Steroid Biochem Mol Biol. 2017 Oct;173:185-193. doi: 10.1016/j.jsbmb.2017.02.005. Epub 2017 Feb 9.
Class 3 semaphorins are transcriptionally regulated by 1,25(OH)2D3 in osteoblasts.
Abstract
The vitamin D endocrine system is essential for calcium metabolism and skeletal integrity. 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]
regulates bone mineral homeostasis and acts directly on osteoblasts. In
the present study we characterized the transcriptional regulation of
the class 3 semaphorin (Sema3) gene family by 1,25(OH)2D3
in osteoblastic cells.
Class 3 semaphorins are secreted proteins that regulate cell growth, morphology and migration, and were recently shown to be involved in bone homeostasis.
In ST2, MC3T3-E1 and primary calvarial osteoblast cell cultures we found that all members of the Sema3 gene family were expressed, and that Sema3e and Sema3f were the most strongly induced 1,25(OH)2D3 target genes among the studied cell types.
In addition, transcription of Sema3b and Sema3c was upregulated,
whereas Sema3d and Sema3g was downregulated by 1,25(OH)2D3 in different osteoblastic cells.
Chromatin immunoprecipitation analysis linked to DNA sequencing (ChIP-seq analysis) revealed the presence of the vitamin D receptor at multiple genomic loci in the proximity of Sema3 genes, demonstrating that the genes are primary 1,25(OH)2D3 targets. Furthermore, we showed that recombinant SEMA3E and SEMA3F protein were able to inhibit osteoblast proliferation. However, recombinant SEMA3s did not affect ST2 cell migration. The expression of class 3 semaphorins in osteoblasts together with their regulation by 1,25(OH)2D3 suggests that these genes, involved in the regulation of bone homeostasis, are additional mediators for 1,25(OH)2D3 signaling in osteoblasts.
Class 3 semaphorins are secreted proteins that regulate cell growth, morphology and migration, and were recently shown to be involved in bone homeostasis.
In ST2, MC3T3-E1 and primary calvarial osteoblast cell cultures we found that all members of the Sema3 gene family were expressed, and that Sema3e and Sema3f were the most strongly induced 1,25(OH)2D3 target genes among the studied cell types.
In addition, transcription of Sema3b and Sema3c was upregulated,
whereas Sema3d and Sema3g was downregulated by 1,25(OH)2D3 in different osteoblastic cells.
Chromatin immunoprecipitation analysis linked to DNA sequencing (ChIP-seq analysis) revealed the presence of the vitamin D receptor at multiple genomic loci in the proximity of Sema3 genes, demonstrating that the genes are primary 1,25(OH)2D3 targets. Furthermore, we showed that recombinant SEMA3E and SEMA3F protein were able to inhibit osteoblast proliferation. However, recombinant SEMA3s did not affect ST2 cell migration. The expression of class 3 semaphorins in osteoblasts together with their regulation by 1,25(OH)2D3 suggests that these genes, involved in the regulation of bone homeostasis, are additional mediators for 1,25(OH)2D3 signaling in osteoblasts.
Muistiin sematoforiinien eräästä osuudesta luun homeostaasiin 25.2. 2019
Semaforiinien
KROMOSOMISIJAINNIT
Kromosomi 1:
SEMA4A (1q22)
SEMA6C (1q21.3)
Kromosomi 2
SEMA4C (3q11.2)
SEMA4F (2p13.1)
Kromosomi 3:
SEMA3B (3p21.31)
SEMA5B, (3q21.1)
SEMA3G (3p21.1)
SEMA3F (3p21.31)
Kromosomi 5:
SEMA5A (5p15.31)
SEMA6A(5q23.1)
Kromosomi
7
SEMA3A (7q21.11)
SEMA3C (7q21.11)
SEMA3E (7q21.11)
SEMA3D (7q21.11)
Kromosomi 9
SEMA4D (9q22.7)
Kromosomi 10
SEMA4G (10q24.31)
Kromosomi 15:
SEJMA6D (15q21.1)
SEMA4B (15q26.1)
Kromosomi 19
SEMA6B (19p13.3)
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